ABSTRACT

Hyperspectral detection in flow cytometry is a powerful tool for over ten multi-color phenotypic analysis without compensation. There are many operating flow cytometers in the marketplace with only 4 or 5 color performance. An objective of this work is to provide a universal hyperspectral detection system to analyze fluorescence signals from a variety of flow cytometers. Using our prototype we were able to evaluate a wide wavelength range (340-800nm) with 42 channels and 16 bit resolution.
The history of this technology can be found here

The first papers published in Spectral Flow Cytometry were papers from the Purdue group. These can be found on the above link.

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